Direct or indirect immunofluorescence: which is best for you?

Direct or indirect immunofluorescence?   Choosing between a primary conjugate (direct) or a secondary antibody (indirect) can make all the difference to your staining. Our guide can help you make the best decision.   Choose your approach       Mouse-on-mouse IHC staining   Staining mouse tissue with a mouse antibody often gives high background. There are ways to reduce this.   Reduce your background   Fluorescent protein properties   Quickly find essential properties like spectra, brightness, and photostability, for some of the most commonly used fluorescent proteins.