A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa | Annals of Clinical Microbiology and Antimicrobials | Full Text

A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa | Annals of Clinical Microbiology and Antimicrobials | Full Text

A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa

• Dhammika Leshan Wannigama, 
• Cameron Hurst, 
• Parichart Hongsing, 
• Lachlan Pearson, 
• Thammakorn Saethang, 
• Naphat Chantaravisoot, 
• Uthaibhorn Singkham-in, 
• Sirirat Luk-in, 
• Robin James Storer & 
• Tanittha Chatsuwan 

Annals of Clinical Microbiology and Antimicrobials volume 19, Article number: 8 (2020) Cite this article

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Abstract

Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.