Gelatin zymography protocol

Hello Victor Norberto

Gelatin zymography protocol   If you need to visualize MMP-2 or MMP-9 activity in your experiments, this could be the protocol for you. In this procedure, active gelatinases digest gelatin embedded in a polyacrylamide gel. After coomassie staining, areas of degradation are visible as clear bands against a darkly stained background. Give it a go and see if this gets you the result you need.