What is Shotgun Transcriptome Sequencing?

What is Shotgun Transcriptome Sequencing?

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By Dr. Sanchari Sinha Dutta, Ph.D.Reviewed by Emily Henderson, B.Sc.

Shotgun sequencing is performed by randomly fragmenting DNA sequences into small pieces, followed by computer-based reassembly of DNA fragments by finding overlapping ends. This technique is used for genomic, transcriptomic, and proteomic sequencing.