herenciageneticayenfermedad: SCF Complex and the Cell Cycle

miércoles, 19 de diciembre de 2018

SCF Complex and the Cell Cycle

	December 19, 2018
	Cell Biology
	The latest cell & molecular biology news from News Medical

Combining Organelle Markers with Defined Isotypes to Achieve Multiplex IHC-IF

One of the ways to visualize several proteins in a single staining is through multiplex immunohistochemistry-immunofluorescence (IHC-IF). Read the full article for the best advice on how to do multiplex IHC-IF with antibodies from the organelle marker panel offered by Atlas Antibodies.

			Getting the Most Out of the CLARITY Technique An interview with Dr. Tim Steppe, discussing the latest advances in the CLARITY technique and his top tips for scientists using this method in their research.

			Conventional Fluorescent Proteins with Integrated Superresolution CLEM By using superresolution light microscopy, we have been able to provide highly accurate correlation of fluorescent proteins with cellular structure.

	The Role of the SCF Complex In The Cell Cycle SCF complexes are a class of E3 ubiquitination ligase that facilitate proteasomal degradation through the catalysis of ubiquitinylation. The cell cycle relies on ubiquitin and the SCF complex plays an integral role in the regulation of this process.

	Researchers attempt to create world's first genetically edited babies The researchers have used CRISPR to edit the genes of IVF embryos in a controversial attempt to create offspring that are resistant to disease. A team of researchers from the Southern University of Science and Technology in Shenzhen plans to eliminate a gene called CCR5 in an effort to ensure the babies are born resistant to HIV, smallpox and cholera.

	Clathrin-mediated Endocytosis In clathrin-mediated endocytosis, the cell membrane invaginates to take up metabolites, hormones, and proteins from the cell surface into intracellular vesicles. Cells employ several pathways for protein and lipid intake from the cell surface. These pathways include micropinocytosis, phagocytosis, and clathrin-mediated or caveolin-mediated endocytosis.

	How to Generate Stable Cell Lines Transfection is a technique used to introduce foreign DNA into eukaryotic cells. Commonly, transfection is used to express a specific protein in cells, either transiently or permanently.

	Inflating cells to analyze organelle structure and function Being able to see these structures would allow an improved understanding of how cells function, but achieving this has been a difficult challenge. Until now, fluorescence microscopy has not provided a resolution high enough to observe the ultrastructure of these components.

	Scientists develop nanoscale tweezers that can move freely within cells Researchers have developed nanoscale “tweezers” that can extract cellular components such as DNA, proteins, and organelles, without causing damage or bursting the cell.